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lenti hiret gfp  (Addgene inc)


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    Addgene inc lenti hiret gfp
    KEY RESOURCE TABLE
    Lenti Hiret Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lenti+hiret+gfp/AAV+phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE+(Plasmid+%2351509)/pmc05679421-621-151-149
    Average 94 stars, based on 65 article reviews
    lenti hiret gfp - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Deconstruction of corticospinal circuits for goal-directed motor skills"

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills

    Journal: Cell

    doi: 10.1016/j.cell.2017.08.014

    KEY RESOURCE TABLE
    Figure Legend Snippet: KEY RESOURCE TABLE

    Techniques Used: Recombinant, Sequencing, Software

    Related Articles

    Recombinant:

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagent will be addressed by the lead author Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 CatN/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: STAR*METHODS KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

    Sequencing:

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagent will be addressed by the lead author Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 CatN/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: STAR*METHODS KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

    Software:

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagent will be addressed by the lead author Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 CatN/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: STAR*METHODS KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

    Bioprocessing:

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagent will be addressed by the lead author Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 CatN/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: STAR*METHODS KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: A sensitized IGF1 treatment restores corticospinal axon-dependent functions
    Article Snippet: KEY RESOURCES TABLE table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab13970 Rabbit polyclonal anti-RFP Abcam Cat#ab34771 Rabbit anti-GFAP DAKO Cat# Z0334 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Rabbit anti-5-HT Immunostar Cat#20080 Rabbit anti-IGFR Santa Cruz Cat#sc-712 Rabbit anti-pIGFRβ Cell Signaling Technology Cat#3024 Rabbit anti-pS6 Cell Signaling Technology Cat#4857 Rat anti-CD68 Bio-Rad Cat#MCA1957 Guinea pig-anti-Vglut1 Synaptic Systems Cat#135304 Goat-anti-ChAT Millipore Cat#AB144P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Quipazine Sigma Cat#Q1004 SKF- 82197 Tocris Cat#1447 8-OH-DPAT Tocris Cat#0529 4-AP Sigma Cat#275875 4-AP-MeOH Santa Cruz Cat# sc-267247 Diptheria toxin Sigma Cat# D0564 Human recombinant IGF1 Peprotech Cat# 100–11 Human recombinant Osteopontin (OPN) Peprotech Cat# 120–35 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Recombinant DNA AAV-PLAP Liu et al., 2010 Cat#N/A AAV-tdTomato Addgene Cat#59462 AAV-Cre This paper Cat#N/A AAV-OPN Duan et al., 2015 Cat#N/A AAV-IGF1 This paper Cat#N/A AAV-CaMKIIa-ChR2-mCherry Addgene Cat# 26975 AAV- Syn-ChR2-EYFP Addgene Cat#26973 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-FLEX-DTR Jin, et al., 2015 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms ImageJ2 NIH https://imagej.nih.gov/ij/index.html Prism 7.0 GraphPad Software, Inc. Cat# http://www.graphpad.com/ STATA 12 College station, TX, USA www.stata.com Other N/A N/A N/A Open in a separate window CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Dr. Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green RetrobeadsTM Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper CatN/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE



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    Journal: Cell

    Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills

    doi: 10.1016/j.cell.2017.08.014

    Figure Lengend Snippet: KEY RESOURCE TABLE

    Article Snippet: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green Retrobeads™ Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper Cat#N/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for reagent will be addressed by the lead author Zhigang He ( ude.dravrah.snerdlihc@eH.gnagihZ ).

    Techniques: Recombinant, Sequencing, Software